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We have compared three rapid DNA extraction processes for direct PCR in ramie (Boehmeria nivea), a fibre crop species containing high amount of gummy complex polysaccharides and developed modified protocols for direct PCR using NaOH as extraction buffer and Tris/Tris HCl/Tris EDTA as dilution buffer.

The modified protocols for rats sensitization and challenge were used as reported previously [ 16].

The modified protocols for mice sensitization and challenge were used as described previously [ 18].

We also established the modified protocols for in situ hybridization and BrdU labeling of bone sections from mice.

Viral RNA was extracted from aliquots of this mixture in triplicate both according to the standard as well as modified protocols for the large volume kit.

We developed and applied modified protocols for plasma DNA extraction, methylated sequence enrichment and sequencing library construction, allowing us to obtain high numbers of good quality unique reads from methylated fcDNA samples.

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Similar(53)

The cell lysate is then assayed using a modified protocol for the LANCE cAMP detection kit.

Images were pre-processed and analyzed using a modified protocol for DTI in SPM2.

A modified protocol for the native purification of the secreted 81 kDa pro-protein of PrtV is provided.

LCM was performed using a modified protocol for human pancreatic tissue dehydration [18], [19].

DNA was purified by use of a modified protocol for Puregene DNA Purification (Gentra Systems, Minneapolis, MN [5].

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