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The apparent improvement in ovarian response in heifers treated with the modified protocols needs to be confirmed in larger field studies.
Decades of studies have led to the development of modified protocols that recapitulate molecular developmental cues by combining various growth factors and small molecules with improved efficiency.
Since the recombinant Δ59 SrtA was first achieved in 1999 using pQE30 (Qiagen) as vector in E. coli (Ton-That et al. 1999), several groups have cloned and expressed SrtA using several commercial plasmids, including pET23b (Novagen) (Guo et al. 2009), pTWIN1 (New England Biolabs) (Bentley et al. 2008) and pBAD (Invitrogen) (Kim et al. 2002), etc., with slightly modified protocols.
Many NDMA protocols, and variations of it, have been proposed and analyzed in the literature, including different ways to determine the number of devices involved in a collision [18 22], interference cancellation receivers [23 25], and modified protocols that use channel knowledge at the transmitter side [26, 27].
We have compared three rapid DNA extraction processes for direct PCR in ramie (Boehmeria nivea), a fibre crop species containing high amount of gummy complex polysaccharides and developed modified protocols for direct PCR using NaOH as extraction buffer and Tris/Tris HCl/Tris EDTA as dilution buffer.
For days, Fernanda persistently tested and modified protocols at various temperatures and lengths of time, until finding the right combination that would allow the perfect reaction to happen.
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This modified protocol is called Ancestors protocol.
A comparative study has been done between ASGK protocol and the modified protocol.
The modified protocol no longer relies on a single layer of authentication.
Lipids were extracted by a modified protocol of Bligh and Dyer (1959) from freeze-dried cells.
We tested the modified protocol using variable neighborhood search (VNS) as a heuristic.
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