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Using this modified protocol, the differentiation efficiency of iPSCs into RPCs and mature renal epithelial cells increased.
According to our modified protocol, the tissue was processed in order to minimize microbial contamination and to remove subcutaneous elements as described earlier [ 18].
When they were resequenced using the modified protocol, the results were identical: 8/8 sequencings displayed complete methylation or complete demethylation at all 20 informative sites.
Using this modified protocol, the coverage rate was found to be high at 98.91%, meeting the recommended coverage by the Joint Committee of Infant Hearing (JCIH) [ 20], and was similar to the coverage rate observed for an obstetric hospital downtown (98.85%), where the majority of expectant mothers were residents [ 21].
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The modified protocol involves the use of a 1 1 mixture of 1% SDS and 8 M urea as protein solubilization solution, and requires sonication.
Thus, the modified protocol of the large volume kit was selected for further evaluation for SARS-CoV RNA extraction from serum.
A modified protocol for the native purification of the secreted 81 kDa pro-protein of PrtV is provided.
To screen for mutations the MassCleave™ a modified protocol from the one originally released by SEQUENOM http://www.sequenom.com was used.
The N-terminus was investigated according to the modified protocol based on the use of the reagent 4-sulfophenyl-isothiocyanate (SPITS) before protein separation by SDS-PAGE electrophoresis.
PKA kinase activities are measured by the cAMP-Glo kit (Promega) with a modified protocol in the in vitro condition.
The DNA was isolated as previously described, 32 using the repeated bead beating procedure with a modified protocol for the QiAamp Stool DNA Mini Kit (Qiagen, Hilden, Germany).
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