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Reducing power was determined following modified protocol reported by [ 25].
The reducing power of A. gracilis samples was determined following modified protocol reported by Saeed et al. [ 11].
The percent inhibition of superoxide anion generation was calculated using the following formula: Percent scavenging activity % = 1 ‒ Absorbance of sample / Absorbance of control × 100 Reducing power of test samples was determined following modified protocol reported by Oyaizu [ 25].
Immediately after surgical resection, solid breast tumor samples were minced and dissociated into single-cell suspensions by incubating at 37°C for 1 h with 1 M HEPES cell dissociation buffer containing 200 U/mL of Liberase TM (Roche) in basic accordance with the modified protocol reported by Panchision et al. [ 7].
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The Comet assay was performed under alkaline conditions following a modified protocol initially reported elsewhere (Singh et al, 1988; Djuzenova et al, 1999, 2001).
This study followed a modified protocol as reported earlier by [ 30, 31] where 1% gelatin has been used to coat culture dishes, which aids in selective removal of fibroblasts due to its higher affinity to a collagenous extracellular matrix like gelatin [ 32].
Colitis was either induced by rectal instillation of 2.5 mg of trinitrobenzene sulfonic acid (TNBS) (Sigma Aldrich Canada Ltd., Ontario, Canada) or mice were fed with 5% dextran sulfate sodium (DSS) (MP Biomedicals, OH, USA) using modified protocols previously reported [46] [47].
This was synthesized by a modified protocol based on previously reported work.
Adipogenesis was induced using the protocol described by Pittenger et al. (1999) [ 40] and a slightly modified protocol of the one reported by Janderová et al. (2003) [ 35].
Adli et al. reported a modified protocol to realize the ChIP-Seq using 10 000 cells by revising the random primers used in amplification to reduce the primer self-annealing, with an optimized PCR condition to cover the GC-rich regions.
Here we report a modified protocol for the purification of T. gondii acidocalcisomes, which provides a fraction suitable for biochemical studies and place the quality of evidence for the presence of acidocalcisomes in T gondii at the level of that from the initial, definitive characterization in trypanosomatids.
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