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Lipids were extracted by a modified protocol of Bligh and Dyer (1959) from freeze-dried cells.
Herein a modified protocol of the FST using within-subject design (repeated rat-FST) was evaluated.
For all genetic manipulations, total streptococcal DNA was extracted using the modified protocol of Miller et al. (1988).
300 mg of milled seeds were then digested using a modified protocol of dry ashing digestion (Jiang et al. 2007).
We designed a modified protocol of PMDAT that evaluates anxiety during retrieval and extinction of the task.
The prosthetic group [18 F]11 was synthesized by following a modified protocol of Li et al. [22] using tetrabutylammonium hydroxide as base during 18 F-fluorination instead of the Kryptofix 2.2.2/potassium carbonate system.
1011 cells were harvested by centrifugation and a nuclear extract was prepared according to a modified protocol of Dignam [45].
To expose rats to stress, we used a modified protocol of an established restraint stress paradigm [12], [13].
To address this issue, we conducted power calculations based on the modified protocol of Hunt et al [18].
SDS-PAGE was performed using a modified protocol of Laemmli [47] made according to instructions for NuPAGE gels (4 12% Bis-Tris, Invitrogen) and PVDF membranes (Millipore).
We then purified the recombinantly expressed fragments and crosslinked them within the range of permissive to non-permissive temperatures (modified protocol of [42]).
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