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We tested the modified protocol in 40 type 2 diabetic patients after elective open-heart surgery (MY group) comparing with 35 type 2 diabetic patients under empirical blood glucose control (EC group).
Total phenol and flavonoid analysis was carried out following the modified protocol in [ 70].
PKA kinase activities are measured by the cAMP-Glo kit (Promega) with a modified protocol in the in vitro condition.
We developed a modified protocol in compliance with the recommended biosafety guidelines from the World Health Organization based on the use of the MagNA Pure total nucleic acid large volume isolation kit for the extraction of SARS-coronavirus RNA.
Myelin staining was done by a modified protocol in potassium dichromate (1%, 24 h), ethanol (25%, 24 h), hematoxylin (0.5% in 25% ethanol, 24 h), and ethanol (25%, 24 h), as described previously [ 32].
The patients who underwent impaction grafting were mobilized according to a modified protocol; in the first 6 weeks, only 10% weightbearing was allowed and then 6 to 12 weeks of 50% weightbearing using two crutches was allowed.
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A slightly modified protocol, described in detail in Tahara et al. and Yee et al., was used to transfect the SLC transporter proximal promoter constructs into HepG2, HCT-116 and ACHN cells.
Stool DNA was extracted under the modified protocol provided in the QIAamp DNA Stool Mini Kit.
This modified protocol resulted in a yield of 5 10 mg of protein per litre of culture and a final product with >95% purity.
If the modified protocol results in a manageable toxicity, this drug combination is a promising treatment option for multiple myeloma patients.
A total of 18 single nucleotide polymorphisms (Barker et al. 2011) were genotyped using the allele-specific primer extension (ASPE) technique, (Luminex, Austin, TX, USA), following the modified protocol outlined in Orantes et al. (2012).
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