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A slightly modified protocol, described in detail in Tahara et al. and Yee et al., was used to transfect the SLC transporter proximal promoter constructs into HepG2, HCT-116 and ACHN cells.
Using a modified protocol described in [ 7], a reservoir filled with lactated Ringers solution was connected to one lateral ventricular catheter while a fluid-filled transducer to measure ICP was connected to the contralateral ventricle by the second catheter.
Silica nanoparticles were prepared according to a modified protocol described previously [23].
The NMD analysis was performed using a modified protocol described by [19].
In addition, casein zymography of VSMC was performed according to the modified protocol described previously [55].
The macrophage killing capacity was quantified by a gentamicin protection assay following a modified protocol described for Salmonella [21].
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Rut-C30 was transformed with the Biolistic®; PDS-1000/He Particle Delivery System (Bio-Rad Laboratories, HerCAles, CA, USA) according to a modified protocol originally described in (Te'o et al. [2002]).
A modified protocol previously described by Ludwig and collaborators [16] of chemically defined hESC medium was applied.
Proteins of different solubility were extracted from brain in buffers of increasing stringency, using a slightly modified protocol previously described [24], [25].
DNA was extracted from the MBC's using a modified protocol previously described by Iovannisci, et al., 2006 [22], using reagents from the MasturePure DNA Purification Kit (Epincentre Technologies, Madison, WI, Cat. No. MCD85201).
Subcellular fractionation was performed using a modified protocol as described (Mapendano et al., 2010).
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