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For ISH, we used a modified protocol based on Strahle et al. [86].
Total RNA was extracted according to a modified protocol based on the manufacturer's instructions (Invitrogen).
CHIP analysis was performed using the modified protocol based on the EZ-CHIP kit (Millipore).
The library construction followed a modified protocol based on Hamilton et al. (1999).
Southern hybridization was performed using a modified protocol based on Sambrook et al. [ 71].
This was synthesized by a modified protocol based on previously reported work.
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Total RNA was isolated from 80
In [47], Ding et al. proposed Vegas-W, which is a modified TCP protocol based on TCP-Vegas [33] for multi-hop ad hoc networks.
The Seahorse XF24 Flux Analyzer (Seahorse) was equilibrated to 37°C overnight and a modified protocol was used based on previous work in mouse liver mitochondria [ 61].
The modified protocol has been proposed based on ASGK with some modifications to balance the number of affected members and the encryption/decryption overhead with any number of the areas when a member joins or leaves the group.
In the present study, we used a modified protocol [ 29] based in the preference for the "old familiar object" over the "recent familiar object" and the preference for a novel location.
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