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[Co en 3]Cl3∙ 3H2O was prepared by a slightly modified procedure described in the literature [81].
Total genomic DNA was extracted from individual mosquitoes following a slightly modified procedure described by Collins [26].
Transmission electron microscopy was performed using a slightly modified procedure described by Tambalo et al. [ 29].
Twenty-five gram leaf tissue was ground into fine powder in liquid nitrogen following the slightly modified procedure described by Luo and Wing [ 28] to release their intact nuclei.
column (modified procedure described elsewhere [ 9 ] -mobile phase: hexane-ethanol-ethanolamine (80:20:0.2, v / v / v ), flow rate (1.0 mL/min), detector UV (295 nm, t = 23 °C).
CIS/ZnS NCs were synthesized according to the modified procedure described in the literature [22].
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The adhesion values of the ETEC strains to IPEC-J2 cells were checked by real-time PCR with slightly modified procedures described by Candela et al.[ 63].
To induce semi-allogenic graft-versus host disease, a slightly modified procedure as described by [ 7] was performed.
The total phenolic contents were estimated according to slightly modified procedure as described previously using Folin Ciocalteu reagent [ 5, 6].
The enzymatic activity of protease was determined using the modified universal procedure described by Sigma-Aldrich with casein as a substrate (Sigma-Aldrich 2013).
After 2 4 weeks, primary cultures were purified by using modified immunopanning procedure described by Mi and Barres (1999) [68].
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