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Based on responses and feedback from the health professionals, we developed a modified pool of 36 items by rewording, combining, or excluding irrelevant items.
The modified pool of 36 items was administered to a convenience sample of 30 parents of children 3 to 5 years of age with a range of dental care needs.
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The design of a new detergent is formulated as a modified pooling problem including process, performance, processability and environmental constraints.
However, the scissile guanine in the anticodon of tRNAPhe carries a post-transcriptional 2'-methoxy modification [ 29], which precludes cleavage of the modified tRNAPhe pool by any RNase producing 2',3'-cyclic phosphate, such as Ire1, leaving only the unmodified pool as a possible substrate.
Therefore a modified oligonucleotide pool is becoming more popular for aptamer selection.
In addition to a modified hydrocarbon pool mechanism, wherein ethene is formed from the lower methylbenzenes, a parallel C3+ alkene methylation/cracking cycle is operative.
Labeling reactions were performed with 4 μg purified amplified DNA and a Bioprime labeling kit (Invitrogen) according to the manufacturer's instructions in a volume of 50 μl with a modified dNTP pool containing 120 μM each of dATP, dGTP, and dCTP; 60 μM dTTP; and 60 μM Cy5-dUTP (for the immunoprecipitated sample) or Cy3-dUTP (for the input sample).
This apparent paradox needs to be considered in the context of observations that BiP oligomerization upon calcium depletion also correlates with less substrate binding (a feature first noted many years ago [ Suzuki et al., 1991] and confirmed here) and with the observation that calcium depletion-mediated oligomerization slowly draws on the modified, inactive pool of BiP.
In fact, with a physical map and BAC end sequences in hand, it may be possible to predict such regions and modify the pool size appropriately.
Little is known concerning the role of soil water flows during rain events in modifying the pool of B. cereus sl strains in soil or concerning the putative transfer of these bacteria to deeper environments and in their fate in groundwater.
Effective conservation management should concentrate primarily on modifying pools' initial properties such as bottom substrate.
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