Sentence examples for modified polyA trap from inspiring English sources
Then modified polyA trap vector containing 24× MS2 tags and Cas9-2sgRNA/PTENP1 were transfected into 293T cells.
For targeted insertion near transcriptional termination site, a modified polyA trap vector containing CMV-puromycin selecassettessette without polyA signal, a specific sgRNA targeting site and 4× MS2 or 24× MS2 tagging sequences were designed (Fig. S1A and S1B).
The remaining five transposons inserted in regions currently without any annotated genes, suggesting either the presence of unannotated genes or the presence of cryptic polyA sequence motifs as the GFP polyA trap was activated.
This construct enables efficient identification of mutant mice in which the transposon has inserted in a gene, as activation of the polyA trap results in ubiquitous GFP expression, i.e., mutant mice fluoresce.
The SB transposon construct (sb-pTrans-SA-IRESLacZ-CAG-GFP-SD Neo) containsb-pTrans-SA-IRESLacZ-CAG-GFP-SD Neo motifsb-pTrans-SA-IRESLacZ-CAG-GFP-SD Neoisb-pTrans-SA-IRESLacZ-CAG-GFP-SD Neo with the gene encoding enhanced green fluoresb-pTrans-SA-IRESLacZ-CAG-GFP-SD NeoG promoter (Horie et al. 2003).
Mice carrying transposon insertions, which activated the polyA trap, were noninvasively identified by visualizing GFP expression in newborn mice under UV light with confirmation by the presence of GFP fluorescence in ear biopsies using a fluorescent stereomicroscope equipped with a UV filter.
They can even be genetically modified to trap CO2 faster, keeping it underground for millions of years.
Circular modified gene trap vector is initially linearized by vector-targeting Cas9-sgRNA complex.
Here we combined CRISPR/Cas9 with modified gene trap vectors for lncRNA tagging and expression manipulation.
Secretions were captured in a modified sinus trap and then transferred into a 0.2 ml microfuge tube using a 1cc syringe.
The modified tissue trap was sealed, and epoxy glue was also applied immediately proximal to the front window to hold a vacuum.
