Sentence examples similar to modified pets and from inspiring English sources

But even in that case, the state Fish and Game Commission based its decision more on an ethical aversion to genetically modified pets than on environmental concerns.

ndk-1 was ligated into a modified pET vector (Novagen) and the GST::NDK-1::His6 protein was expressed in E. coli strain Rosetta pLysS.

The wt-ERα LBD (wild-type) and the ERα-Y537S LBD mutant (amino acids 302 552) were cloned into a modified pET-15b vector, and the wt-ERβ LBD (amino acids 261 502) was cloned into a pET-32a vector.

PCR fragments were cloned into a modified pET vector via BamHI and XhoI restriction sites.

PCR amplicons encoding FMRP Nt-KH1 and FXR1P Nt-KH1 were cloned into a modified pET-24 plasmid (Novagen) with NcoI and NotI sites and containing an N-terminal His6-Trx (thioredoxin) tag with a cleavage sequence for TEV (tobacco etch virus) protease ENLYFQ* GA (the asterisk indicates the cleavage site and the extra residues left after cleavage are underlined).

After sequence verification, the 5' and 3' fragments were retrieved and ligated with a modified pET vector as a SUMO fusion with an N-terminal 6xHis-tag.

The PLCζ1 307/386 647 was amplified further from pCR3-PLCζ1 307/386 647 to incorporate a 5′ SalI and 3′ NotI site, and subcloned into a modified pET vector (pETMM30) to enable bacterial expression.

The PLCζ/XYlδ1480 491 was amplified further from pCR3-PLCζ/XYlδ1480 491 to incorporate a 5′ SalI and 3′ NotI site and subcloned into a modified pET vector (pETMM30) to enable bacterial expression.

The leucine zipper from yeast GCN4 peptide (residues: 250 281) was cloned between BamHI and BglII sites into a modified pET expression vector which allows N-terminal GST fusion and cleavage by TEV protease.

Wild-type human PLCζ (GenBank #AF532185) and the H233L and H398P mutants were amplified by polymerase chain reaction (PCR) from the corresponding pCR3 plasmid by use of Phusion polymerase (Finnzymes) to incorporate a 5′ SalI site and a 3′ NotI site and were cloned into a modified pET expression vector (pETMM60).

The DNA sequence encoding residues 131 458 (N-terminus) and 736 1200 (C-terminus) of mDia1 was amplified by PCR and ligated into a modified pET vector containing a His6 tag with a TEV cleavage site.