Sentence examples similar to modified pet and from inspiring English sources
ndk-1 was ligated into a modified pET vector (Novagen) and the GST::NDK-1::His6 protein was expressed in E. coli strain Rosetta pLysS.
PCR fragments were cloned into a modified pET vector via BamHI and XhoI restriction sites.
After sequence verification, the 5' and 3' fragments were retrieved and ligated with a modified pET vector as a SUMO fusion with an N-terminal 6xHis-tag.
The PLCζ1 307/386 647 was amplified further from pCR3-PLCζ1 307/386 647 to incorporate a 5′ SalI and 3′ NotI site, and subcloned into a modified pET vector (pETMM30) to enable bacterial expression.
The PLCζ/XYlδ1480 491 was amplified further from pCR3-PLCζ/XYlδ1480 491 to incorporate a 5′ SalI and 3′ NotI site and subcloned into a modified pET vector (pETMM30) to enable bacterial expression.
The leucine zipper from yeast GCN4 peptide (residues: 250 281) was cloned between BamHI and BglII sites into a modified pET expression vector which allows N-terminal GST fusion and cleavage by TEV protease.
Wild-type human PLCζ (GenBank #AF532185) and the H233L and H398P mutants were amplified by polymerase chain reaction (PCR) from the corresponding pCR3 plasmid by use of Phusion polymerase (Finnzymes) to incorporate a 5′ SalI site and a 3′ NotI site and were cloned into a modified pET expression vector (pETMM60).
The DNA sequence encoding residues 131 458 (N-terminus) and 736 1200 (C-terminus) of mDia1 was amplified by PCR and ligated into a modified pET vector containing a His6 tag with a TEV cleavage site.
The PfCelTOS insert was subcloned into a modified pET(K−) expression vector (Novagen, Madison, WI), via a 5' BamHI and a 3' NotI site, creating the pET(K− PfCelTOS (3D7) plasmid.
On the other hand, GloFish, the world's first genetically modified pet, got quick F.D.A. approval in 2004.
Ageing of the modified PET was accompanied by an increase of the contact angle which was due to a reorientation of the molecular polar segments produced during the plasma treatment.
