Sentence examples for modified nucleotides are from inspiring English sources

We demonstrate that these modified nucleotides are efficiently incorporated by DNA polymerase, and the DNA strand bearing biotinylated nucleotides is captured by streptavidin-coated beads and efficiently released using tris 2-carboxyethyl phosphine in aqueous solutris 2-carboxyethyl phosphineh DNA and downstream procedures.

Such modified nucleotides are relatively easy to synthesize and incorporate in DNA strands.

Chemically modified nucleotides are restored by base excision repair (BER).

In ribosomal RNAs, modified nucleotides are located mostly in regions corresponding to the functional centers of the ribosome [ 11- 14].

We conclude that these modified nucleotides are incorporated during the IVT reaction at ratios which are reflective of the input ratio to the unmodified counterpart.

Beside C* in the minor tRNA-Ile (C*AU) and U* in several U34-containing tRNAs, the chemical structures of all modified nucleotides are known.

Helm, M. et al. The presence of modified nucleotides is required for cloverleaf folding of a human mitochondrial tRNA.

The modified nucleotides were mostly found in the single stranded region of the RNA which validates the MFOLD prediction.

Amino-allyl modified nucleotides were incorporated during the aRNA synthesis (2.5 mM rGAU (GE Healthcare), 0.75 mM rCTP (GE Healthcare), 0.75 mM AA-rCTP (TriLink Biotechnologies).

Chemically modified nucleotides were identified by primer extension.

Amino-allyl modified nucleotides were incorporated during the overnight in vitro transcription step according to the manufacturer's protocol.