Sentence examples for modified nucleotides and from inspiring English sources

This included the synthesis of random libraries with modified nucleotides and the enzymatic amplification of SELEX pools that contain modified nucleotides (see Materials and Methods).

Cluster 16 is a heterogeneous group of receptors binding to lipids, nucleotides, modified nucleotides and platelet activating factor (PAF).

Epigenetic factors include several different classes of modified nucleotides and proteins that interact to regulate the activity state of underlying DNA sequence.

The most widely used sequencing platforms in genetic research today are the massively parallel sequencing platforms, which have inherited many features from Sanger sequencing, such as the use of polymerases for synthesis, modified nucleotides and fluorescent detection.

Contemporary tRNAGly contains on average the fewest post-transcriptionally modified nucleotides (and the lowest proportion of modifications at position 37, immediately downstream of the anticodon, important for stabilizing the anticodon-codon interaction through base stacking [ 66]), with the unmodified state assumed to be ancestral [ 44].

To our surprise, salt uptake rather than hydration appears to play a dominant role in the specificity of mCpG discrimination, electrostatics play an outsized role in binding to modified nucleotides, and the domain can assemble on DNA to form a discrete dimer.

With respect to the first hypothesis, we examined how putative RNA modification sites may affect the relationship between miRNAs and their targets, assuming that the pairing between a modified nucleotide and its target nucleotide can be mimicked by pairing of the substituted nucleotide and the target (Fig. 5).

From ten separate SELEX experiments with seven diverse modified nucleotides, including single and double ring aromatics as well as fluorobenzyl and tyrosyl groups, only one SELEX experiment yielded a high-affinity ligand.

Giese and co-workers have developed a method for nontargeted analysis of modified nucleotides in DNA (and RNA) involving derivatization of the nucleotides with isotopologue benzoylhistamines followed by MALDI-TOF or MALDI-TOF/TOF analysis.

MMR proteins recognise mismatched base pairs in the DNA, arising either spontaneously during DNA metabolism or from modified nucleotides provoked by physical and chemical agents, and are thought to link DNA damage recognition to an apoptotic pathway, thereby preventing mutagenesis, tumorigenesis, and tumour progression (Modrich, 1991; Fink et al, 1998).

Termini modified nucleotides amplify the signal and allow the examination of labelled cells under microscopy, flow cytometry, or immunohistochemistry [ 10].