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Bjork, G. R. Genetic dissection of synthesis and function of modified nucleosides in bacterial transfer RNA.
The most frequently used method for the incorporation of modified nucleosides in synthetic RNAs is via solid-phase oligonucleotide synthesis based on phosphoramidite chemistry.
In the presence of Na+, the incorporation of two, three or five modified nucleosides in the third strand has improved the efficacy of formation of the triplex as compared to that formed with the unmodified oligonucleotide.
21 modified nucleosides in tRNAs were identified in both species.
By contrast, the study of tRNA modified nucleosides in plants has rarely been documented.
Homozygote lines were isolated and modified nucleosides in total tRNA were subsequently analyzed.
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In fact, the wobble position is the most frequently modified nucleoside in tRNA.
The base-pairing of a natural base with the modified nucleoside in the oligomers during DNA synthesis will cause the replacement of its equivalent partner.
Cutoff level was the average mean concentrations+standard deviations of each modified nucleoside in the urine of the 41 healthy volunteers.
Based on the tRNA modification candidate genes found in Arabidopsis (Table 3), we decide to use publically available T-DNA mutant lines to identify genes specific for each modified nucleoside in Arabidopsis.
HPLC chromatograms of the two species were very similar; all modified nucleosides present in Arabidopsis were also present in hybrid aspen, with only slight differences for the relative abundance of some peaks.
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