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The study, published in the journal Science Advances and presented at the American Association for the Advancement of Science conference, describes how researchers modified nematode worms to develop Alzheimer's-like symptoms, and then applied the existing anti-cancer drug, bexarotene, at various stages of the disease.
Animals were cultured and all crosses performed at 20° with the bacterial strain OP50 on modified nematode growth medium containing 1% agar and 0.7% agarose to prevent burrowing of strains with the CB4856 version of npr-1.
All nematode strains were cultured and maintained at 20°C on modified nematode growth media (NGM, 0.35% peptone) agar and fed with E. coli strain OP50, as described (Brenner, 1974).
C. elegans wild-type Bristol N2 animals and mutants were maintained as hermaphrodites at 20°C, grown on modified nematode growth medium (NGM) agar plates, and fed with E. coli strain OP50 as described [23].
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These genetically modified nematode- and crown gall-resistant walnut plants could help reduce the demand for environmentally harmful nematicides.
The unique features of this highly organized intestinal epithelium, coupled with the ability to quickly generate genetically modified nematodes in the lab, provide significant potential advantages for studying intestinal inflammation, compared with the use of more complex model systems (McGhee, 2007).
For this purpose we employed genetically modified C. elegans nematodes expressing GFP in VD-Type motoneurons and pharyngeal nerve ring neurons.
The cadavers of potential interest were surface-sterilised in 0.5 to 1% NaOCl for 3 5 s, washed in water, then individually placed onto a nematode trap modified as per White (1927).
Identification of these proteins will provide further insight into how formation of a complex extracellular matrix, such as the nematode cuticle, has been modified to suit the requirements of the organism.
[ 7] and [ 9], however, developed an agar-gel technique for recovering nematode larvae from herbage samples, and this technique was later modified by [ 17] to isolate nematodes from the gastro-intestinal tract of sheep.
For rhabditids and diplogasterids androdioecious nematodes, hermaphrodites are basically modified females that produce a limited number of sperm.
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