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Cryopreservation was performed using slightly modified methods described by Avarbock and colleagues [18].
Enzyme activities were determined spectrophotometrically at 25°C using modified methods described (Kohn et al., 2011a).
Phenyltrialkylborate tetramethylammonium salts listed in Scheme 1 were prepared in my laboratory with modified methods described by Neckers et al. [ 11, 12].
RT-PCR was performed according to the modified methods described by Gentsch et al., [ 20] and Gouvea et al., [ 21] (Table 1) using 30 thermal cycles at 94°C for 1 min, 55°C; for 2 min, 72°C for 2 min followed by 10 min incubation at 72°C.
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NFAP2 was purified from this mycelia-free supernatant based on the slightly modified method described at NFAP previously (Virágh et al. 2014).
Dried tuber powder (5 g) was treated with slightly modified method described by Drapeau et al. [ 12].
Screening of the fungi for oil yield was carried out using a slightly modified method described by [ 62].
Coniferyl alcohol was used to prepare a calibration curve, and the results are reported as equivalents of coniferyl alcohol [ 29] Pectins were measured using modified method described by Melton and Smith [ 30].
Most of the requisite 5-substitued oxindoles were prepared by modifying methods described in the literature [26, 29 35] or were obtained commercially.
The RNA extracts of cells and tissues were prepared according to the modified method as described.
The total antioxidant activity of test sample was measured with modified method as described previously [ 16].
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