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DNA was extracted following a slightly modified method used for the black tiger shrimp [43].
DNA was extracted from the tissue using a Qiagen Plant Mini Kit, MoBio Ultra Clean Soil DNA Kit (following the manufacturer's instructions), or a slightly modified method used for black tiger shrimp [ 32, 56].
Aliquots of frozen powder (100 mg) were extracted based on a modified method used in Bowne et al. (2012).
The modified method used for selective determination of mineral As species in CCA-contaminated soils was found to be quantitative and reliable.
Statistical methods were categorized by a modified method used by Emerson [13].
As temperatures have increased over the past five decades, and are predicted to continue rising [26], the modified method used here includes all months with a mean temperature greater than 10°C.
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A decellularised human amniotic membrane, prepared by a novel modified method using a combination of wetting agent, alkali and enzyme, is able to retain its physical and biological properties as a suitable extracellular matrix with good biocompatibility.
Our modified method uses primers containing extended non-overlapping sequences at the 3' end (significantly larger than suggested in [ 13]) and primer-primer complementary sequences at the 5' end.
A comparison of the original and modified methods using a subset of 86 isolates indicates little difference in overall locus diversity, discriminatory power or in the clustering of strains within Neighbor-Joining trees.
The main objective of this study was to test these modified methods using case reports.
Opal hydrogels of PS- co-PDMAA spheres could detect SCN− ions in the concentration range from 10 10 nmol/g 301 Modified methods using the opal hydrogel PhCs were developed for the detection of Hg2+ 302 and ionic strength.
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