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Exact(5)
The decision was to ask for a modified manuscript in which these issues are addressed, either by toning down the language, providing amplified discussions of the caveats and limitations of the structural studies, or providing biochemical evidence.
We would be prepared to accept a modified manuscript that softens the conclusions – say by "evidence for" or "suggest" before the declarative statement in the title and related softened statements in the abstract.
The modified manuscript will definitely provide good insight into the parallel evolution leading to similar changes at identical sites and the evidence for weak negative selection on these sites in proteins.
We have moved it forward in this modified manuscript so that it is now associated with Figure 1 and is referred to as Figure 1 figure supplement 4 (Detection of Gal4DBD binding).
NP: designed and performed experiments, interpreted results, drafted and revised the manuscript; YM, CS, AYL performed experiments, interpreted results, drafted the manuscript; NK performed experiments using animals; SEU and CZ designed and supervised the research, analyzed the data and wrote and modified manuscript.
Similar(55)
MCh modified the manuscript and drafted the manuscript.
SZ designed and carried out the experiments, analyzed the data, and wrote the paper; ZZ helped modify the manuscript; LK designed the experiments and modified the manuscript.
VD and AdB drafted the manuscript, EJM, MHN, MET added to and modified the manuscript.
BZ modified the manuscript.
KMC and WQL modified the manuscript.
VBT modified the manuscript and supervised all the projects.
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