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The method yields stably modified lines suitable for long term studies of Leishmania virulence, relies solely on host rather than introduced genetic machinery, and is thus readily applied in many species and circumstances including functional genetic testing.
With respect to rice genetic improvement, developing NILs is the optimal approach to obtain modified lines with desired traits.
Genes' expression involved in SL biosynthesis (D27, D17, D10, Os900 and Os1400), perception (D14 and D3), and signalling (D53) were detected among different genetical modified lines.
Then CKs content were measured by MetWare (http://www.metware.cn/) based at ABSciexQTRAP®4500LC-MS/MS ABSciexQTRAP®4500LC-MS/MSgenetically modified lines.
Additionally, we also detected the content of four CKs (iP, tZ, cZ and DZ) in tiller basal portion among different genetically modified lines.
Interesting, 1000-grain weight among all genetically modified lines exhibited slight decreased compared with that in WT (Fig. 1u), which indicated that appropriate expression of OsNPF7.2 might be beneficial to increase grain weight.
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That led to a phone conversation with General Surovikin in which the two commanders agreed on a slightly modified line.
The strategies range from modified line-girder analysis to 3D finite element approaches.
Two vessel segmentation approaches are applied, using the standard line operator and a novel modified line operator.
The effects on spatial attention were examined separately for the two hemi-spaces by means of a modified line-bisection task.
Then, from the SMP, we derive a set of optimization problems that constitute MLA: Similarity index problem, product grouping problem (PGP), and modified line segmentation problem (LSP).
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