Sentence examples for modified intein expression from inspiring English sources

These results indicate that a modified intein expression system can be used to produce pharmaceutical peptides in transgenic plants.

The modified intein contained a Bacillus circulans chitin-binding domain which allowed binding of the fusion protein on a chitin column and could be induced to undergo in vitro peptide bond cleavage which specifically released Cre from the column.

In agreement with the results in E. coli, all of the modified inteins when expressed in yeast displayed high efficiency splicing.

When expressed in E. coli, all of the modified inteins spliced at high efficiency.

For biotechnological purposes, modified inteins have been designed.

The modified inteins with the 4 different selection marker cassettes also showed highly efficient splicing.

To determine whether the modified inteins would function in yeast we constructed a new panel of yeast CEN4/URA3 plasmids that contained the marked inteins expressed under the control of the GAL1 promoter.

In pilot experiments all four modified inteins showed high splicing efficiency, both in E. coli and yeast, and in yeast the proteins within the excised inteins were active and enabled growth under conditions of selection.

Based on the limits of detection in the anti-GFP Westerns, we estimate that the efficiency of splicing is greater than 96% for the modified inteins.

We have streamlined this process by introducing Ligation-Independent Cloning (LIC) capability to our intein expression plasmids, which provides a simple method for constructing self-cleaving tag-target gene fusions.

Taken together, the evolved yeast intein platform provides a robust alternative to bacterial intein expression systems.