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For example, H3K9Ac, H3K4me3, H3K27me3, and H3K9me3 are histone modifications that can be found at promoter regions, whereas other modified histones are associated with enhancer regions (e.g H3K4me1) or with transcribed units (e.g. H3K36me3).
This could point to a scenario whereby patterns of modified histones are specified by complex mechanisms.
An emerging body of evidence indicates that post-translationally modified histones are primary targets for autoantibodies in patients with rheumatic disease.
Several studies suggest that SETD2 and modified histones are linked to transcriptional activation and repression, DNA repair, and cell cycle regulation.
This removes the need to optimize the chromatin to antibody to beads ratios as a suitable number of modified histones are present as an epitope for the antibody.
Site-specifically modified histones are crucial reagents for structural analysis of PTM binding modules that are sensitive to the nucleosomal context.
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He explored how modified histones were copied when a cell divides, right down to the molecular level.
With the exception of H3K9Me1, the modified histones were localized to the nuclear periphery.
Probes that were associated with modified histones were called on the basis of a neighbourhood p-value of less than 0.05.
Antibodies for modified histones were also obtained from Abcam.
Antibodies for modified histones were used at 1 µg/ChIP reaction.
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