Sentence examples for modified gene trap from inspiring English sources

Circular modified gene trap vector is initially linearized by vector-targeting Cas9-sgRNA complex.

Here we combined CRISPR/Cas9 with modified gene trap vectors for lncRNA tagging and expression manipulation.

In principle, modified gene trap vector and Cas9-2sgRNA were transfected simultaneously into 293T cells for donor DNA plasmid linearization and targeted insertion at desired genome locus (Fig. 1A).

As gene trap system has been well-established to disrupt gene functions with selection markers/tags for subsequent functional analysis (Stanford et al., 2001), we here modified gene trap vectors and used CRISPR/Cas9 to establish a scalable tool entitled CTRL (CRISPR-mediated tagging and regulation of lncRNAs) for lncRNA tagging and expression manipulation in mammalian cells.

CTRL system contains a modified gene trap vector, a plasmid expressing S. pyogenes Cas9 (SpCand) and two sgRNAs driven by two U6 promoters respectively (one genome-targeting sgRNA and another donor plasmid-targeting sgRNA) (defined as Cas9-2sgRNA) for lncRNA tagging and expression manipulation purposes.

The modified gene trap is highly mutagenic at molecular and phenotypic levels, resulting in isolation of two embryonic lethal and one post-embryonic lethal mutations which are revertible by Cre-mediated recombination.

Another danger of the project is the possibility of having a modified gene show up in other bacteria or insects.

Allerca screened thousands of cats to identify a population with the modified gene and then set those cats to breeding.

"Tell us the gene you want modified and we will give you the modified gene with the full analysis," Dabrowski said.

They then put these modified genes back into peanut plants.

One of the more compelling uses would put modified genes into wild populations of organisms.