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The modified gene model file was added to the bitbucket repository subdirectory titled BlastxAnalysis/results as V_corymbosum_scaffold_May_2013_wDescr.bed.gz.gz
One such modified gene model is commd10 (DDB_G0275249): the gene prediction used an incorrect splice site.
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339 (35%) of modified gene models showed a net increase in total exons while 126 (13%) showed a net decrease.
Of the 974 modified gene models, 453 (30%) had no error flags within their FDM sequences, but showed a more that 10% increase in gene size.
For the 974 significantly modified gene models, it is useful to look more closely at the nature of the modifications made.
Of the set of 1501 gene models, 1439 (96%) generated modified gene models from BES's in which all error flags were successfully spanned and sequences aligned to genomic sequence.
A vast majority of the Myc-BestModel gene models (1439 or 96%) generated modified gene models with all error flags were successfully spanned and transcript sequences well aligned to genomic sequence.
Of the set of 1501 gene models, 1439 (96%) successfully generated modified gene models in which all error flags were successfully resolved and the sequences aligned to the genomic sequence.
The final output of this method is the set of expressed BES gene models in FASTA format, the structural annotation of the gene models in GFF format, and a new set of FDMs sequences resulting from the re-alignment RNA-seq data to the modified gene models using 'Bowtie'.
One additional interesting category of modified gene models are those which contain no deviations from observed expression data within the boundaries of the BestModelv1 gene models, but nonetheless showed significant changes to gene structure by addition of up and/or downstream expressed sequence.
In total, 1529 modified gene models are presented in the RRes v4.0 genome annotation.
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Justyna Jupowicz-Kozak
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