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DNA fragments were blunted using NEBNext® End Repair Module (NEB; E6050L) as per the manufacturer's instructions but with a reaction volume modified for the volume of DNA.
Total protein quantitation was performed using the Bradford method 11 modified for low volume and high throughput.
Genomic DNA was extracted starting from young leaves of O. europaea cv "Leccino" following the nuclei extraction protocol of Zhang et al. (1995), modified for small volumes.
Activity assays were performed according to the same protocol; however, the method was modified for a total assay volume of 200 μl which allowed for sample measurement in a 96-well microplate.
modified for suction, and growth chamber volumes were maintained at ∼25 ml with some fluctuation.
Glucokinase (GK) enzyme activity was measured as previously described [ 12] however; solution volumes were modified for a 96-well plate and ODs were measured on a plate reader (BioTek Instruments, Inc. Winooski, VT, USA).
The assay was slightly modified for this experiment by using a larger reaction volume (2 mL total volume), which accommodates multiple sampling over time.
It is interesting that the modified USY zeolite has the highest secondary pore volume when it was modified for 3 or 5 h.
For the IG-IMRT group, plan was modified for the last 5 fractions (10 Gy) with reduced PTV volume.
The CS acquisition scan pattern can be easily modified for different percentages of missing data which corresponds to the duration of a volume acquisition.
We have modified for this purpose the equation proposed by Farina et al. for the conductive volume used to model the striated muscle surface EMG [ 9].
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CEO of Professional Science Editing for Scientists @ prosciediting.com