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Additionally, glutamate oxidase was also modified for the detection of Glu.
The developed nanocluster/enzyme signal probe can certainly be modified for the detection of different other trace targets in bioanalytical fields.
Alamethicin has been modified for the detection of protein ligand interactions and poly ethylene glycol) polymers.
The MSAP method [ 76], modified for the detection of CpG methylation in S. mansoni[ 13], was applied to platyhelminth gDNA samples free from host contamination (Additional file 2).
The general immunohistochemistry protocol was modified for the detection of BrdU, adding a DNA denaturation step with 2 N HCl (30 min, 37°C), as previously described (Gomez-Nicola et al., 2011, 2013).
In oral fluid specimens, PRRSV-specific IgA and IgG antibodies were detected using IgA- and IgG-based IPMAs, immunofluorescence assays (IFA), a commercial IgG-based ELISA (Idexx PRRS OF) and a commercial PRRSV ELISA modified for the detection of PRRSV-specific IgA in swine oral fluid [ 12].
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Furthermore, the RT-LAMP procedure was modified for the direct detection of HIV-1 nucleic acid in plasma and blood samples, eliminating the need for an additional nucleic acid extraction step and reducing the overall procedure time to approximately 90 min.
The nitroblue tetrazolium (NBT) staining method of Rao and Davis (1999) was modified for the in situ detection of superoxide radicals [58].
The electrochemical capability of the fabricated GNP-MWCNT composite modified electrode for the detection of 5-FU is examined by cyclic voltammetry, electrochemical impedance analysis and differential pulse voltammetry.
The validation of the DNP and FBS assays was conducted in parallel with the published methods devised by Green (both unmodified version for the specific detection of artesunate [15] and modified version for the detection of artesunate, artemether and DHA [16]).
The validation of the DNP and FBS assays was conducted in parallel with the published methods devised by Green (both unmodified version for the specific detection of artesunate [15] and modified version for the detection of artesunate, artemether and DHA [16] and sulphuric acid assay [10]).
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