Sentence examples for modified for dna from inspiring English sources

Their DNA was isolated using the phenol/ chloroform/ isoamyl alcohol method, modified for DNA isolation from filamentous fungi, as described previously [ 144].

Highly purified fungal genomic DNA was isolated from mid-exponential cells grown in YNB media with 20% (w/v) NaCl, using the phenol/ chloroform/ isoamyl alcohol method, modified for DNA isolation from filamentous fungi, as described previously [ 61].

The ICR-2LS software package (23 ) was used to deconvolute the mass spectra and calculate the mass of the monoisotopic species using an "averagine" fitting routine (24 ) modified for DNA (Drader et al., unpub. data).

Using synthetic oligonucleotides modified for DNA methylation analysis, we determined that the CpG island associated with miR-125b1 is methylated in most breast tumor cell lines, with the exception of SK-BR-3.

DNA from archived scale samples were extracted using a phenol-chloform extraction procedure modified for "ancient" DNA [ 34].

DNA fragments were blunted using NEBNext® End Repair Module (NEB; E6050L) as per the manufacturer's instructions but with a reaction volume modified for the volume of DNA.

The NGS MP protocol provides a method to specifically interrogate a gDNA sample for large rearrangements and was thus modified for the input WGA DNA.

To adapt these real-time PCR assays for more resource-poor laboratory settings, a PCR-restriction fragment length polymorphism (RFLP) assay was developed and primer/probe combinations were modified for use in oligochromatography, a DNA 'dipstick' technology.

After verifying Pax8 target enrichment, IP and non-IP DNAs were modified for sequencing following the ChIP-Seq manufacturer's protocol (Illumina, San Diego, CA).

Adult participants will undergo a medical assessment appropriate to their group (gynaecologic evaluation, and infectious disease evaluation for HIV-infected participants), cytological screening following the Italian Guidelines [ 31] for Cervical Cancer or algorithms modified for HIV-infected women, and HPV DNA testing.

By altering the buffer volumes and the incubations the modified protocol allows for DNA extraction of a complete blood spot within a single reaction column without sacrifice of DNA yield.