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The protein content in each sample of cell lysate was determined by the method of Lowry [ 13] modified for analysis in 96-well plates.
Tissue supernatants were analysed for AChE activity using the method described by Ellman et al. (1961), modified for analysis in a 96-well plate reader.
Finally, after addition of internal standards (20 ng each of deuterated-PCB 30 and 204), samples were analyzed by gas chromatography with tandem mass spectrometry (GC-MS/MS, Agilent 6890N Quattro Micro GC, Waters Micromass MS Technologies) in multiple reaction monitoring mode, using a method derived from EPA Method 1668c, but substantially modified for analysis with the aforementioned instruments.
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A commercially available molecular beam mass spectrometer modified for biomass analysis was used for pyrolysis vapor analysis [ 44– 44].
Weighted gene co-expression network analysis was performed as described previously [ 37, 38] using an R-script modified for this analysis and a network threshold cut off of 0.01 (Additional File 4).
In order to compute the optimal dowel length in cement concrete pavement, semi-infinite beam on elastic foundation was deduced and modified for the analysis of dowel bars.
Single-pellet moment technique which was initially proposed for the measurement of effective diffusivities in porous catalysts and adsorption rate and equilibrium constants was modified for the analysis of gas-solid reactions.
Exploiting the nature of DTI that it is calculated from multiple diffusion-weighted images of each region, permutation testing, a non-parametric hypothesis testing technique, was modified for the analysis of serial DTI data and implemented for voxel-wise hypothesis tests of diffusion metric changes, as well as for suprathreshold cluster analysis to correct for multiple comparisons.
The NSS method was modified for the analysis here in two critical ways.
Both the programs have been modified for the analysis of coding sequences.
Some of the original diet score categories were modified for our analysis.
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