Sentence examples for modified expression strategy from inspiring English sources

To overcome the toxicity of expressed protein, we modified expression strategy to slow down protein expression by growing the cells at 30°C in Luria-Bertani growth medium containing 1% glucose and induction with 0.1 mM IPTG for 3 h, which resulted in highly efficient induction of protein in the cell lysate at 50 kDa [Fig. 1b, left panel, encircled].

Strategies to effectively direct MSCs to sites of injury and inflammation including genetic manipulation, modifying expression of homing and adhesion receptors, and antibody or peptide-directed cell targeting [ 110] are actively being pursued.

Functional delivery of oligonucleotides to epidermis in order to edit or modify expression of mutant genes.

For vector-based shRNA, improvements can be achieved through manipulating expression strategy, using different types of promoters, modifying shRNA structures, and simultaneously expressing multiple shRNAs in a single vector [ 9– 13].

The expression strategy included using a previously reported modified α-factor preproleader for enhanced secretion and pAOX1, a methanol-responsive promoter.

The modified strategy of constitutive expression of shRNA and inducible expression of AGO2 in a single lentiviral-vector has advantages of a low toxicity and prolonged efficacy.

The microbial version of the gene encoding the protein was modified in order to achieve two expression strategies in transgenic alfalfa plants.

Here the modified TEE strategy dominated the manual palpation strategy.

In the present study we have therefore modified our strategy.

* In this scenario the modified TEE strategy dominated the manual palpation strategy.

Our results indicated that the common commercial vectors, such as pET30a, could be modified and optimized for a particular co-expression strategy in E. coli.