Exact(4)
Finally, the three entry clones, in combination with the modified destination vector, were incubated to create a BHD-pDESTR4R3 targeting construct through BP recombination reaction.
All Gateway®Entry and modified destination vectors were propagated in DH5α (Invitrogen, UK).
The resulting clone was, together with AtCHR23 cDNA entry clone described above, assembled in a multi-step LR Gateway reaction into the modified destination vector pBGW (http://gateway.psb.ugent.be/).psb.ugent.be/
The entry clone was used in a second site-specific recombination reaction with the modified destination vector pcD3 (origin pcDNA3) to generate the expression clone (following the standard protocol, as described by Invitrogen).
Similar(56)
In research of WMNs' routing mechanisms, previous work mostly focuses on modifying the existing ad hoc routing mechanisms while this paper focuses on modifying destination-sequenced distance-vector routing mechanism (DSDV) with cross-layer design method and proposes the modified destination-sequenced distance-vector routing mechanism (MDSDV) for WMNs.
These modified pGWB1 destination vectors now have Gateway cassettes after the pSPA1 or pSPA4 promoters, respectively.
Fragments of Ana2 were PCR amplified from cDNA and subcloned into modified pRNA destination vectors (Conduit et al., 2014) using the Gateway Life Technologiess, Carlsbad, CA) system.
Third, the modified pGWB1 destination vectors described above were recombined with the Entry clones containing the ORFs from AtSPA1, OsSPA1, PpSPAb and AtSPA4, respectively, using Gateway LR technology to generate pSPA1 AtSPA1/OsSPA1/PpSPAb vectors and pAtSPA4::AtSPA4/PpSPAb vectors.
Also the association between age and campylobacteriosis was modified by travel destination, and in travellers from tropical destinations, especially from Africa and Asia/Oceania the highest risk was seen in the youngest children (Table 3).
After receiving the modified PREQ, the destination mesh router will choose the malicious mesh router as the next hop in the reverse path and unicast PREP to the malicious mesh router.
shRNA cassettes were recombined by Gateway cloning into a modified pLenti-6 destination vector (pGT3, Invitrogen) to generate lentiviral shRNA expression constructs shCtrl1, shCtrl2, shTP53_1, shTP53_2, and shTP53_3.
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