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Furthermore, the algorithm is applied to analyze influenza virus replication in genetically modified cell lines.
The latter was created using parental and genetically modified cell lines.
The reporter gene bioassays are based on genetically modified cell lines, which have been stably transfected with specific reporter genes (e.g. firefly luciferase).
In order to alleviate this step, we developed a strategy aimed at the rapid and efficient generation of genetically modified cell lines with conditional, inducible expression of various target genes.
We confirmed specific functionality of the encoded R77H variant by replicating impaired phagocytosis in genetically modified cell lines.
These observations regarding patterns of co-expression are in agreement with the previously described mutual dependence model from modified cell lines.
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To test that notion, the team used a genetically modified cell line that expressed the receptor for FasL found on T lymphocytes and mixed in FasL-bearing astrocytoma cells from mice, rats, and humans.
hChonJb 7 is genetically modified cell line produced via retro-viral infection and recombination.
Statistical comparison of each Snail2 modified cell line and their appropriate control cell line was performed using the Student's t test in Microsoft Excel.
Cells treated with HGF/SF and Adriamycin also had modified cell line regulation and signal transduction that might suggest mechanisms by which HGF/SF exerts its protective activity [ 31].
Strikingly, we observed enhancement in the rate of growth of EGFR-p53DD-PTEN−/− cells as compared with the various double-modified cell lines.
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