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To test that notion, the team used a genetically modified cell line that expressed the receptor for FasL found on T lymphocytes and mixed in FasL-bearing astrocytoma cells from mice, rats, and humans.
hChonJb 7 is genetically modified cell line produced via retro-viral infection and recombination.
Statistical comparison of each Snail2 modified cell line and their appropriate control cell line was performed using the Student's t test in Microsoft Excel.
Cells treated with HGF/SF and Adriamycin also had modified cell line regulation and signal transduction that might suggest mechanisms by which HGF/SF exerts its protective activity [ 31].
However, these techniques require the use of a modified cell line for all screening purposes.
The modified cell line displayed a doubling time of 12 hours in culture and a plating efficiency of 34%.
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The following CRISPR modified cell lines made at the DRSC are available from the Drosophila Genome Resource Center DGRCC) in Bloomington, IN.
The latter was created using parental and genetically modified cell lines.
Furthermore, the algorithm is applied to analyze influenza virus replication in genetically modified cell lines.
In order to alleviate this step, we developed a strategy aimed at the rapid and efficient generation of genetically modified cell lines with conditional, inducible expression of various target genes.
The reporter gene bioassays are based on genetically modified cell lines, which have been stably transfected with specific reporter genes (e.g. firefly luciferase).
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