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Using a modified cell fractionation protocol that was first developed by da Cunha e Silva et al. [19], flagella detached from cell bodies and therefore free of associated basal bodies or bi-lobes were purified from T. brucei.
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Thus, we used cell fractionation to prepare nuclear and cytoplasmic fractions from DU145 cells.
Membrane and cytosolic fractions were isolated using the QProteome cell fractionation kit (Qiagen; Crawley, United Kingdom).
For subcellular distribution between cytoplasm and nucleus, cell fractionation was performed by differential centrifugation.
A pioneer of modern cell biology used cell fractionation and electron microscopy to describe subcellular structures.
(J) Translocation of Drp1 to the mitochondria, as measured by cell fractionation.
Cell fractionation was performed as previously described, with minor modifications (Li et al. 2010a, b).
Cell fractionation experiments were performed to evaluate the extent of cellular and nuclear uptake of 111In-BnDTPA-F3 111In-BnDTPA-F3 111In-BnDTPA-F3
The localization of l-asparaginase was carried out using cell fractionation techniques.
Cell fractionation included isolation of the cytoplasm, periplasm, membrane, outer membrane vesicles, and extracellular media.
Isolation and purification of mitochondria was performed by optimized cell fractionation method.
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