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The detailed protocol of the method termed antigen-mediated genetically modified cell amplification (AMEGA) is described.
To address this problem, we have developed the antigen-mediated genetically modified cell amplification (AMEGA) system, which uses chimeric receptors to enable the selective proliferation of gene-transduced cells.
In this study, we propose an antigen-mediated genetically modified cell amplification (AMEGA) system employing an antibody/receptor chimera that triggers a growth signal in response to a non-toxic hapten dimer.
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Genetically modified cells may require special conditions.
Five tumor-bearing KPC mice generated a total of 168 single CTCs that were subjected to a modified single-cell amplification and library protocol (Tang et al., 2010), followed by a screen for RNA quality (Gapdh, Actb).
DMSO did not modify cell viability.
Zanet, J. et al. Endogenous Myc controls mammalian epidermal cell size, hyperproliferation, endoreplication and stem cell amplification.
Complex background sample was prepared from Human Leukemia, Chronic Myelogenous (K-562 cell line) poly A+ RNA (BD Biosciences, Palo Alto, CA) by a modified Eberwine amplification technique producing biotin-incorporated cRNA.
*1 array discarded due to poor hybridization Total RNA from the BT474 breast cancer cell line and StratRef was linearly amplified through two rounds of in vitro transcription (IVT) based on a modified T7 amplification[ 19].
The methods discussed include modified gene amplification methods, modifying vectors to improve expression of the therapeutic gene and improving the method of selecting for high-producing cells.
We found that the tissue-specific pattern of gene expression is not modified upon amplification.
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