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We have modified cell adhesion assay techniques5,11 to provide a sensitive measure of cell substrate adhesion for both wild-type and variant HTC cells in the presence and absence of serum and plasminogen.
Cell adhesion to a matrix was determined with a modified cell adhesion assay [76].
Endometrial cancer cell lines that had undergone EMT accompanied by modified cell adhesion molecules and cytoskeleton reorganization were found to have upregulated ETV5 [ 14, 29].
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In addition, a wide range of proteins modifies cell adhesion complexes by controlling the structure or activation state of their constituents [4].
Our finding that MEs are overrepresented in association with these genes indicates that periconceptual environmental influences may be important in modifying cell adhesion in humans.
Cells, which were cultured without animal-derived agents and also treated with a proteolytic enzyme to transiently modify cell surface adhesion proteins, were infused 24 h after transient middle cerebral artery occlusion (MCAO).
Cells treated with a proteolytic enzyme (pronase) could transiently modify cell surface adhesion proteins [ 168].
N2 supplement, which contains transferrin, was used in the presence of antibody to minimize the possibility that antibody protein non-specifically modified cell-substrate from adhesion and therefore neurite outgrowth.
This hydrogel system was prepared by first fabricating a bimodal hydrogel in which the microscale, alginate gel blocks modified with cell adhesion peptides containing Arg-Gly-Asp sequence (RGD peptides), and those free of RGD peptides, were alternatingly presented.
Photocrosslinkable polyvinyl alcohol hydrogels that can be modified with cell adhesion peptides for use in tissue engineering.
It is concluded that n-6 essential fatty acids have the ability to inhibit both motility and invasiveness of human colon cancer cells, perhaps by modifying cell-surface adhesion molecules.
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