Sentence examples for modified by site from inspiring English sources

These plasmids were modified by site directed mutagenesis to introduce unique ClaI (−45) and EcoRI(+20) sites flanking tDNAAla.

Subsequently, the 5 additional Leu codons were modified by site directed mutagenesis, using primer pairs containing unique restriction sites to facilitate mutant's identification, i.e. AccI (Leu20), AvaI (Leu53), HindIII (Leu129, Leu134), and XhoI (Leu155).

We first generated a proVEGF-C mutant (proVEGF-C mut) in which the wild type PC cleavage site was modified by site directed mutagenesis from HSIIRR into HSIISS as previously described (6).

NO2 showed significant spatial variability, predicted by population density and roadway length within 50 m of the home, modified by site characteristics (obstruction), and with higher concentrations during summer (R2 = 0.56).

These plasmids were modified by site directed mutagenesis and adaptor insertion (see Material and Methods, Figure 1A) to allow the easy one step cassette cloning of recombinant antibodies extracted from a large collection of common recombinant antibody selection and expression plasmids (e.g pHEN, pSEX, pHAL, pCANTAB, pHOG, pOPE, pSTE).

To improve the yield further, the gene Hpchyb from Haematococcus pluvialis was modified by site-directed mutagenesis to increase the enzyme efficiency or/and to prevent the heterologous protein degradation by ubiquitination.

Key amino acid residues of the salicylate 1,2-dioxygenase (SDO), an iron (II) class III ring cleaving dioxygenase from Pseudaminobacter salicylatoxidans BN12, were selected, based on amino acid sequence alignments and structural analysis of the enzyme, and modified by site-directed mutagenesis to obtain variant forms with altered catalytic properties.

The gene for ricin toxin A chain was modified by site-specific mutagenesis to change arginine 180 to alanine, glutamine, methionine, lysine, or histidine.

Histones can be epigenetically modified by site-specific combinations of phosphorylation, acetylation and methylation, which correlate with specific biological readouts, such as transcriptional activation or repression, chromatin remodelling or stabilization [32].

The native ELAC2 expression plasmid was modified by site-directed mutagenesis using the QuikChange (Stratagene) protocol: GTCACC replaced (i) the natural 5' untranslated region, or (ii) the 5' untranslated region plus the first 45 nucleotides of coding sequence.

The fosB minigene was modified by site-directed mutagenesis in such a manner that the PTB binding site within the 3' end of I4 was eliminated, but the pyrimidine-rich nature of the sequence was maintained (Fig. 4A, ISS/T6).