Samples from which mycobacteria were isolated were reanalyzed retrospectively by real-time PCR with modified primers and probes targeting the ITS region as previously described (18 ).
The adhesion values of the ETEC strains to IPEC-J2 cells were checked by real-time PCR with slightly modified procedures described by Candela et al.[ 63].
The motility of invasive cells within the 3D Matrigel was monitored by real-time imaging with a modified epi-illumination Zeiss microscope (Zeiss, Oberkochen, Germany) equipped with a Hamamatsu CCD camera (ORCA-AG; Hamamatsu Photonics, Hamamatsu city, Japan).
Toxigenicity was verified by real-time PCR (8 ) and a modified Elek test (6 ).
TREC concentration was assessed by real-time PCR protocol as described [41] and modified by us [42], [43].
After administration of genetically modified fibroblasts, distribution of the plasmids in the peripheral tissues or organs was detected by real-time PCR.
Independent confirmatory experiments by real-time RT-PCR or other techniques are clearly advisable for any microarray results obtained with a modified measurement or analysis protocol [8].
In HDSS, the modified SAGE analysis constructs the gene expression profiles with 18-bp tags, with which we can easily proceed to the second screening by real-time quantitative RT PCR.
The samples were examined by real-time PCR in a 7900HT Real Time PCR System (Applied Biosystems).
This was confirmed by real-time PCR.
