Sentence examples for modified by incubating from inspiring English sources

Tryptophan residues were modified by incubating purified enzyme with increasing concentrations of NBS (0.1 1.0 mM) in 50 mM of sodium citrate buffer, pH 4.5 at room temperature.

Tyrosine residue were modified by incubating purified enzyme with increasing concentration of N-acetyl-imidazole (10–50 mM) in 50 mM Sodium borate buffer, pH 7.6.

The plasmid isolation procedure was modified by incubating the cell suspension in solution I containing 50 µg ml−1 lysostaphin (Sigma) for 1 h at 37°C.

Approximately 500 ng of genomic DNA was bisulphite modified by incubating at 98 °C for 10 min and 64 °C for 2 h and 30 min.

Briefly, VN was modified by incubating the protein (10  μg/ml) with MGO (500  μM) in 100 mM sodium phosphate buffer, pH 7.4, at 37 °C for 72 h.

For assessing the optimal assay temperature and pH, the assay conditions were modified by incubating the reaction mixture at 25, 30, and 40 °C and dissolving starch in either 100 mM acetate buffer (for pH 4.0, 4.5.0,.0, and 5.5) or 100 mM phosphate buffer (for pH 6.0 and 6.5).

To demonstrate attachment to other solid supports a beaded agarose affinity support (Affi-Gel 102 resin, Bio-Rad) was modified with oligoglycine by incubating the amino-resin with diglycine (0.5 M) and EDC (2.5 mM) for three hours at 50°C.

The modified residue was identified by incubating the three samples [unmodified Cg10062, (R)- 6-modified Cg10062, and (S)- 6-modified Cg10062] with endoproteinase Glu-C (protease V-8) and analyzing the resulting peptide mixtures by MALDI-MS.

The non-specific protein interactions of modified SPE were minimized by incubating with blocking solution for 30 min.

The thiol modified oligonucleotide is reduced by incubating with 0.1 M DTT in 0.18 M phosphate buffer pH 8 for 1 h.

Purified RT-PCR products were modified with A-overhangs by incubating with 10 mM dATP and 1u of Taq polymerase (Promega, Madison, USA) in 1× manufacturer's buffer for 10 mins at 72°C.