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Pair-wise mannered alignments were generated with Clustal W program (MEGA 4) [19] and slightly modified by eye.
These sequences were aligned by CLUSTAL X (Thompson et al. 1997) and the alignment was further modified by eye.
Sequence alignments for each locus were initially produced in Sequencher 5.0 or SATé-II [ 24] using MAFFT aligner and OPAL merger and further modified by eye.
Amino acid sequences were aligned using MUSCLE 3.7 (Edgar 2004), on the EMBL-EBI server using default parameters, and modified by eye to minimize indel regions.
Nucleotide and protein sequences were aligned with CLUSTALX (Thompson et al. 1997) and modified by eye with Bio-Edit (Hall 1999).
The obtained ITS sequences and known sequence of Halophila decipiens Ostenfeld (KC175913) and H. minor (AF366405; AF366406) were aligned by CLUSTAL X [ 42] and the alignment was further modified by eye.
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Deduced amino acid sequences of 90 putatively functional V2R genes (including family C V2Rs) in zebrafish, medaka, fugu, and pufferfish and several related GPCRs (CaSRs, GPRC6As, and T1R taste receptors in some vertebrates) were aligned by using the program FFT-NS-i (MAFFT 5.731) [ 50] and slightly modified by eyes.
We investigated further whether the CLN3 overexpression phenotype in the eye is modified by key regulators of the stress response.
Overexpression of Bchs in the Drosophila eye causes a reduced eye phenotype, which is modified by mutations in genes thought to be involved in autophagy (Lim and Kraut, 2009; Simonsen et al., 2007).
Fossorial animals represent natural models of dysgenic eyes in which molecular pathways involved in eye development have been modified by natural selection.
What the eye sees is not modified by the Englishman for the sake of harmonious order, which on the contrary is essential to Claude's Classicism.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com