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The C/C surface was modified by direct reaction with a transition metal.
Firstly, the binding of TFs could be blocked by MBPs at promoter regions due to methylation; secondly, when TF has a chance to bind to its target, the binding stability could be modified by direct interacting with the methylated DNA sequences; thirdly, after a gene transcription had been initiated, the elongation could be blocked by MBPs bound on gene body due to DNA methylation.
In order to increase the wettability of CFC by molten copper, the composite surface was modified by direct reaction with group VI transition metals which form a carbide layer and allow a large reduction of the contact angle.
The joining technique is based on the direct copper casting on CFC surface, which was previously modified by direct reaction with a transition metal of the VI B group.
A protocol for data treatment is proposed and applied to a XPS study of the degradation induced by X-ray on high-density polyethylene surfaces modified by direct fluorination.
The second protocol consisted of five therapeutic immunisations with LM-Mb modified by direct incorporation of αGC (I- αGC-LM-Mb).
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Prior to metal deposition, TiO2 was chirally modified by the direct anchoring of cinchonidine (CD) using trimethoxysilane as coupling agent (TMS-CD).
The few bladelet tools, however, differ from those of Pego do Diabo in that, except for a couple of inversely retouched Dufour bladelets, they are all of the Font-Yves type, i.e., straight and pointed, as at Pego do Diabo, but modified by bilateral direct retouch instead of alternate retouch.
These plasmids were modified by site directed mutagenesis to introduce unique ClaI (−45) and EcoRI(+20) sites flanking tDNAAla.
We first generated a proVEGF-C mutant (proVEGF-C mut) in which the wild type PC cleavage site was modified by site directed mutagenesis from HSIIRR into HSIISS as previously described (6).
Subsequently, the 5 additional Leu codons were modified by site directed mutagenesis, using primer pairs containing unique restriction sites to facilitate mutant's identification, i.e. AccI (Leu20), AvaI (Leu53), HindIII (Leu129, Leu134), and XhoI (Leu155).
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