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All EEGs were classified by severity based on the predominant waveform from grade I to IV with I° = theta-delta-rhythm, II° = delta rhythm, III° = triphasic waveforms, and IV° = burst-suppression (modified as previously reported [ 16]).
The insulin secretion assay method was modified as previously reported [13, 21].
Sprouting assays were modified as previously reported (Korff and Augustin, 1999).
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The as-yet uncultured candidate bacterial genus "Candidatus Entotheonella" of the marine sponge Theonella swinhoei from Japan have recently been recognized as the source of numerous pharmacologically relevant polyketides and modified peptides, as previously reported by the Piel group (Wilson et al. 2014).
GO was synthesized using a modified Hummers method as previously reported.
Protein concentration was measured by a modified Lowry method as previously reported [ 27].
Functional studies in fibroblasts: Human Dermal Fibroblasts (HDF) were grown in Dulbecco's modified essential medium as previously reported [ 15].
Therefore, we modified our criteria as previously reported [ 34], possibly underestimating the incidence of AKI by missing patients with transient decrements in urine output.
In addition, the slices were stained with the modified Bielschowsky silver method as previously reported [ 1].
The genomic DNA was extracted from peripheral blood samples using modified salting-out procedure as previously reported[ 14].
Inihibin A circulatory concentrations were measured using a modified two-site immunoassay (ELISA) as previously reported (McNeilly et al., 2002), using an inhibin βA subunit monoclonal antibody as the capture antibody (Groome et al., 1990) and an αC-specific biotinylated monoclonal antibody (Bleach et al., 2001).
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