Bone marrow cells (BMCs) were obtained from tibiae and femura from C57Bl/6 and Balb/c wild-type mice according to the method of Dobson et al [37], and expansion was started at a concentration of 2×106/55 cm2 Petri dishes in 10 ml Dulbecco's modified Eagle's minimal essential medium (DMEM, Perbio, Bonn, Germany) modified as described by others [20].
The difference maps were modified, as described in the next section, to generate a final Q x filter based on monopole volumes [see panels (c) and (d) in Figures 7, 8 and 9].
First fermentations were carried out in synthetic grape must MS300 (Salmon and Barre [1998]), which was modified as described by Rossouw and Bauer ([2009]) and designated as RB-SGM.
Applying the guidelines for diagnosis of intraocular tuberculosis that were defined by Gupta et al. [2] modified as described in the "Methods", all 49 cases were classified as "presumed" tuberculous optic neuropathy.
It results in the difference maps as a function of binned volume pairs [see panels (a) and (b) in Figures 7, 8 and 9]. iii) The difference maps were modified, as described in the next section, to generate a final Q x filter based on monopole volumes [see panels (c) and (d) in Figures 7, 8 and 9]. .
Items (vi) and (vii) were modified as described below.
Genomic DNA from HCC tumors and surrounding tissue was extracted and modified as described above.
For this purpose, the process described in Villanueva et al [21] was modified as described below.
We used whole-mount ex ovo avian embryo culture initially described by New [50] and modified as described in [44] to secure the quail embryo to its vitelline membrane.
With this strategy it is possible to study and to compare these results with other epithelial networks, including those derived from pathological situations in which the thymic architecture was modified as described in autoimmune and infectious diseases.
Its energy function was modified as described above with the HINT energy term, whose weight, wHINT, was manually optimized (to minimum Rfree) by performing refinement with incremental values between 10 and 100 (the trivial wHINT = 0 case is the native CNS protocol).
