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Workflows will need to be modified and standardized.
We modified and standardized for our specific purposes a previously reported sperm preparation protocol for FISH analysis [ 31].
An Early Treatment Diabetic Retinopathy Study grading system that has been modified and standardized in other population-based studies was used for the diagnosis of diabetic retinopathy (22, 23).
To investigate bacterial migration from patients to the hand of the surgical staff through micro-perforations in gloves, the modified and standardized Gaschen-Bag method was used as previously described [ 9].
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To determine baseline status and study chronic effects, one uses standardized and modified health questionnaires and standardized pulmonary function.
Using Michigan Department of Health resources and local and federal funding, Kendrick and Eldering developed standardized diagnostic tools; modified and improved extant vaccines; conducted the first successful, large-scale, controlled clinical trial of pertussis vaccine; and participated in international efforts to standardize and disseminate the vaccine.
We used a modified ATS standardized respiratory symptom questionnaire (Ferris 1978), translated into Chinese and back-translated into English, to collect information on work, medical, and smoking history.
In fact, they displayed different staining patterns from those obtained with the modified TRIzol protocol, and with the mirVana™ PARIS™ and standardized laboratory protocols.
Decree modified, and, as modified, affirmed.
For measuring the mobility of the MTP1 the protocol, as used in the study of Hopson et al. [ 10], was slightly modified (not standardizing the step length) and used in this study, see Additional file 3: Video 3.
Our results demonstrated that the steady-state expression levels of β-actin and GAPDH obtained with the modified TRIzol protocol are very similar to those obtained with mirVana™ PARIS™ and standardized laboratory protocols.
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