Sentence examples for modified an amplification from inspiring English sources

We have modified an amplification procedure that requires only 1 μg of total RNA.

Primers for COII amplification were forward modified A-tLeu 5'-CAGATAAGTGCATTGGATTT-3' and reverse B-tLys 5'-GTTTAAGAGACCAGTACTTG-3' [ 48, 49], modified by Miura et al. [ 16].

For generation of libraries for Illumina sequencing from pools of sorted EGFP-positive astrocytes, we modified an established linear amplification protocol using the DNA-dependent RNA polymerase from bacteriophage T7 (Rossner et al. 2006).

Complex background sample was prepared from Human Leukemia, Chronic Myelogenous (K-562 cell line) poly A+ RNA (BD Biosciences, Palo Alto, CA) by a modified Eberwine amplification technique producing biotin-incorporated cRNA.

Complete 5′ and 3′ terminal nucleotide sequences of the 11 gene segments were determined by using a modified rapid amplification of cDNA ends technique as described (15 ).

*1 array discarded due to poor hybridization Total RNA from the BT474 breast cancer cell line and StratRef was linearly amplified through two rounds of in vitro transcription (IVT) based on a modified T7 amplification[ 19].

In this study, we propose an antigen-mediated genetically modified cell amplification (AMEGA) system employing an antibody/receptor chimera that triggers a growth signal in response to a non-toxic hapten dimer.

G4-stabilisation was assessed using a high-throughput FRET (fluorescence resonance energy transfer) assay and telomerase inhibition quantified by a modified TRAP telomerasee repeat amplification protocol) method.

Telomerase activity was assessed using a modified telomeric repeat amplification protocol (TRAP) assay, in accordance with the manufacturer's instructions (BD Science).

RNA (2 5  μg) was linearly amplified using a modified amplification procedure originally described by Van Gelder et al (1990).

Forty-five of the viruses, which included viruses isolated in July, were tested at NICD by using a modified amplification refractory mutation system PCR (ARMS-PCR) (7 ).