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To quantitatively evaluate the Ca2+ content in the ER and its permeability, we modified a protocol from a previous study conducted in sympathetic neurons (Albrecht et al. 2002).
We modified a protocol described previously [38].
For this purpose we used a modified a protocol originally developed for metabolite measurements in vascular bundle cells of A. thaliana [ 15] (Fig. 1).
In light of these observations, we modified a protocol from Pauklin et al. (Pauklin and Vallier, 2013), in which the cells were treated with nocodazole for 16 hr, washed to remove the drug, and then treated with aphidicolin for 3 hr before nucleofection.
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The following method was developed by modifying a protocol previously reported for other squid species [ 35].
Constraint: We modified an existing protocol 99 to suit our population and study focus.
The PFGE protocol was modified from a protocol originally developed for Campylobacter spp. [ 23].
Protein aggregates were isolated by differential centrifugation by modifying a described protocol [47].
A FA PEG RAFT macroCTA was synthesized by modifying a previous protocol.
The Caco-2/DCs co-culture system was developed modifying a published protocol (Rescigno et al, 2001).
Capped mRNA was synthesised using the SP6 Megascript kit (Ambion) and a modified protocol using a 1∶10 ratio of GTP to m7G(5')Gppp(5')G cap.
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