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After two rounds of homologous recombination, engineered C. glutamicum with the corresponding chromosomal modifications were verified by PCR.
The modifications were verified by DNA sequencing (in-house ABI lab DNA sequencing core facility, Dept. Molecular Biosciences, University of Oslo).
Modifications were verified as appropriate via subsequent patient interviews and no new relevant themes or issues were highlighted.
All constructs were generated by restriction and modification enzymes and all cloning modifications were verified by sequencing.
Peptide identification and the presence of their covalent modifications were verified by inspection of parent mass fragmentation patterns using programs MassLynx (Waters) and ProteinProspector.
Our modifications were verified and described by Cardosa and colleagues[ 24].
Similar(54)
The modifications are verified using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM).
These modifications are verified by metallographic observations of the deformed coating near the indentation print and introduced in two other models.
The sequence resulting from the genetic modifications was verified by DNA sequencing.
The amino acid position of missense variants in functional domains, disulfide bonds, or posttranslational modifications was verified, as well as their effect on protein tridimensional structure, when available, using NCBI Protein (http://www.ncbi.nlm.nih.gov/protein) and UniProtKB (http://www.uniprot.org/).org/
Putative phosphopeptides (neutral loss of H3PO4) reported by Mascot searches ("Phosphorylation (ST)" was used as variable modification) were verified by manual inspection of the corresponding MS2 spectra.
More suggestions(15)
alterations were verified
modifications were made
modifications were validated
adjustment were verified
modifications were completed
modifications were explored
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modifications were discussed
modifications were included
modifications were examined
modifications were considered
modifications were calculated
modifications were observed
modifications were followed
modifications were preserved
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