Exact(4)
The modifications were as follows.
The modifications were as follows: first, mutation distance d was distributed obeying binomial distribution; second, the selection process obeyed the natural selection protocol; third, ruggedness was introduced to the landscape according to the NK model; fourth, a noise was included in the fitness measurement.
Assay modifications were as follows: Platinum Taq DNA Polymerase (Invitrogen, Brazil) was used as the master mix, and for the rrs assay the Mg concentration was reduced from 4.50 to 4.25 mM, the forward primer reduced from 1 µM to 0.25 µM, the reverse primer reduced from 1 µM to 0.50 µM and the probe reduced from 0.20 µM to 0.05 µM.
Modifications were as follows: All solutions were prepared using diethylpyrolecarbonate (DEPC) treated water.
Similar(4)
When it comes to maintaining genomic integrity, epigenetic changes such as those involving DNA and histone modifications are as profound as the genetic ones.
Modification is as follows.
The mechanism of stepwise modification is as follows.
Plasma density of the inductively-coupled RF plasmas, which were used for modification, was as high as 1013 cm− 3 at the plasma generation region.
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