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To examine p53 modifications we treated cells with PXD101, doxorubicin, or Nutlin-3A.
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Table 3 summarizes the elemental modifications on treated nylon and PES membranes.
These modifications were treated at two levels, which consists in: (I) using either a double- or a triple-zeta valence basis set; (II) including or not diffuse functions in basis set, (III) including or not polarization functions in basis set, and (IV) performing a calculation with or without electron correlation beyond the Hartree Fock level, i.e., MP2 or B3LYP.
However, the two modifications are treated as independent in the model.
The topic of post-translational modifications is treated comprehensively by Len Neckers and Mehdi Mollapour in this special edition on Hsp90 in the chapter titled "Post-translational modifications of Hsp90 and their contributions to chaperone regulation".
We next examined the bulk histone modifications in cells treated with DZNep, TSA or both.
As early as 30 minutes post treatment with either drug, there was a significant decrease in phosphorylated serine 473 AKT and by three hours post treatment we were unable to detect this post-translational modification in treated osteosarcoma cells.
The improvements in hardness and corrosion resistance are discussed considering microstructural modifications in the treated samples.
While dietary modifications were not treated as CAM in this study, they are discussed separately.
Considering the overall outcome, the decellularization process leads to limited modifications of the treated tissues compared to native ones.
These modifications can be treated as testable hypotheses for which experiments can be designed to prove/disprove their validity.
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