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To explore age-related metabolic modifications, we measured biochemical and hematological parameters in the three mouse strains on alternate months for one year.
H3.3 dissociation rates varied greatly among histone modifications: we measured the highest dissociation rates at euchromatic marks such as H3K4me3, H3K9ac, and H3K27ac.
In order to better characterize the gene- and locus-specific early and late methylation modifications, we measured the methylation level at specific CG sites in SRC, PLEKHG6 and ITGB2, which showed a huge erasure of DNA methylation (approximately 70%to80%0%) either from monocytes to iDCs or from iDCs to mDCs during this time course.
To test whether Snail-1 binding is associated with time-dependent chromatin modifications, we measured log2 ratio changes in the chromatin marks H3K4Me1, H3K4Me2, H3K4Me3, H3K27Me3, H3K4Ac, and H3K27Ac, all within −2 kb to +2 kb of the TSS for Snail-1 regulated transcripts.
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To ensure the integrity of each specimen and the formation of Au-S bond on GNR after MUA modification, we measured the characteristic extinction spectra, the XPS, and the zeta potential of as-synthesized GNR, GNR-MUA, and 1-undecanethiol modified gold nanorods (GNR-UDT) (Figure 1c).
To indirectly assess whether BTRC or an unidentified regulator decreased Mcl1 stability through altered post-translational modification, we measured the survival of Mcl1 protein following inhibition of protein synthesis 48 h after siRNA knockdown.
To confirm that certain patients did not show a change in acetylation, we measured histone modification changes alternatively by western blot analysis.
To establish whether the enhanced expression of genes playing a role in the immune system are due to modifications in the microbiota we measured the total number of all bacteria and of the two most prominent phyla colonizing the colon, Bacteriodetes and Firmicutes, in the luminal content of the colon.
To test whether IRF2 facilitates histone modifications at the TLR3 promoter, we measured H3K4me3 and H3K9/K14ac using ChIP assays.
To test if reduced SOD2 activity plays a role in increased nitrosative stress and therefore in the observed peroxynitrite-mediated PS changes, we measured nitrotyrosine modifications in brains from Sod2+/− mice.
We measured histone modification and RNAPII complex levels during serum stimulation in a ChIP assay (Fig. 5).
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